Lipid Production from Crude Glycerol by Newly Isolated Oleaginous Yeasts: Strain Selection, Molecular Identification and Fatty Acid Analysis

Biodiesel is a renewable alternative fuel and glycerol as main byproduct of the manufacturing process. Lipids could be produced from crude by using yeasts. The ability 107 yeast strains to utilize was screened 92 these were selected. 60 determined potential for lipid production Sudan Black B staining. After secondary screening 25 them showed specific growth rates (OD 600), high biomass content. These identified Pichia cactophila, P. fermentans, anomala, Rhodotorula mucilaginosa, R. dairenensis, Clavispora lusitaniae, Saccharomyces cerevisiae, Wickerhamomyces anomalus, Candida glabrata, C. inconspicua, albicans, Yarrowia lipolytica with molecular identifications based on ITS D1/D2 26S rDNA sequences. results that cactophila accumulated up 64.94%, highest C16:0, C18:0, C18:1 C18:2 essential fatty acids biodiesel detected GC–MS in lipids all strains. lusitaniae reported first time lipid-producing suggest selected isolates have grow especially produce has use feedstock second generation production.